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hrp  (Bio-Rad)


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    Structured Review

    Bio-Rad hrp
    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), <t>and</t> <t>HRP-conjugated</t> secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.
    Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+anti+rabbit+hrp+conjugated/Rabbit+anti+Sheep+IgG+(H%2FL)/pmc13006224-67-22-30
    Average 92 stars, based on 55 article reviews
    hrp - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA"

    Article Title: Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2026.1775573

    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.
    Figure Legend Snippet: Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Techniques Used: Recombinant, Titration, Concentration Assay

    Related Articles

    other:

    Article Title:
    Article Snippet: The secondary antibody used was sheep anti-rabbit HRP conjugated from BioRad, this product has been discontinued.



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    Image Search Results


    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Journal: Frontiers in Veterinary Science

    Article Title: Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA

    doi: 10.3389/fvets.2026.1775573

    Figure Lengend Snippet: Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Article Snippet: Optimization parameters included: (i) coating concentration of the single antigen or antigen mixture, (ii) serum dilution, and (iii) working dilution of the HRP-conjugated secondary antibody (Rabbit anti-sheep IgG (H/L): HRP; BIO-RAD, 5184–2,504).

    Techniques: Recombinant, Titration, Concentration Assay